Thermo multiskan fc manual

The side view of the The Procedure Desktop controls the instruments and allows basically limitless assay setup according to the users needs. In the uncommonly flexible Results Desktop, measurement data is reduced, results calculated and reports created Clear and easy-to-follow, this software features a highly-visual assay protocol setup, user-friendly data handling and effective integration to automation and LIMS systems..

Thermo Multiskan Ascent Plate reader with Ascent Software never used, still in the box, with software and cables. Other items in same category. Labsystems …. Ascent software for multiskan manual Thermo Scientific Multiskan FC - Filter based microplate photometer for research applications and routine microplate photometer The Thermo Scientific Multiskan FC is a reliable and robust instrument for a wide range of research activities and routine applications.

It has been designed to give you the information …. The Multiskan EX is a basic reader for endpoint and kinetic assays. Part of Thermo …. This effect is shown in Fig. Ideal curve Actual curve due to shorter height of column of the concave liquid surface Standard curve due to negative lens effect of the concave liquid surface Fig.

This effect arises from the fact that the concave surface functions as a negative lens Fig. The cone of the light leaving the surface will be wider, and some of the light is diverted enough to bypass the detector. Surfaces with identical curvature will cause equal losses of light at the detector and equal increase in the apparent absorbances at all absorbance values. The absorbance will be increased by an additive amount, and the "standard curve" will have the same slope as the actual curve, but intersects the absorbance axis above the origin as shown in Fig.

The correction is made by using the blank cuvette block with a similar surface to the analysis cuvettes. Because of the difference in the slopes obtained by flat and concave surfaces it is important to have identical shapes of liquid surfaces in all cuvettes including blank, standard s and samples.

This is achieved by using the following standardization and calibration procedures: 1. In kinetic methods, i. Therefore it is necessary to use the corrected molar absorptivity coefficient to obtain accurate results.

The corrected values of molar absorptivities are provided in the Multiskan EX applications. Sends measurement data and status information to an external printer. Receives commands from the external computer. Sends data, acknowledgements and status, including appropriate error messages.

The Multiskan EX in-built software comprises program modules, which are combinations of different measurement and calculation modes. See Table 4. The keys available are presented in Table 4. In equations, first press the shift key, then the letter. Press first the shift key. See Sections 6. The instrument returns to the previous logical state save To store user-determined programs in the memory of the instrument recall To recall programs stored earlier meas mode To select the measurement mode meas param To enter the measurement parameters calc mode To select the calculation mode calc param To enter the calculation parameters param To select the general parameters, e.

Refer to the enclosed packing instructions. Keep the original packaging and packing material for future transportation. Use of alternative packaging materials may invalidate the warranty. To lift the instrument, put your fingers under the bottom on both sides and lift it with your back straight, taking proper precautions to avoid injury.

The net weight of the instrument is 11 kg 24 lbs. Check the enclosed packing list against order. In case of any deviations, contact your local Thermo Fisher Scientific representative.

Visually check all interconnections in the basic instrument. Check that there are no loose parts inside the instrument. Refer to Section 5. Avoid sites of operation with corrosive vapors, smoke and dust, vibrations, strong magnetic fields, direct sunlight, draft, excessive moisture or large temperature fluctuations. Leave sufficient clearance at least 10 cm on both sides and at the rear of the unit. Warning: DO NOT operate the instrument in an environment where potentially damaging liquids or gases are present.

See Section 5. Install the lamp. Caution: DO NOT touch or loosen any screws or parts other than those specially designated in the instructions.

Doing so might cause misalignment and will invalidate the instrument warranty. See Fig. DO NOT insert the computer's serial cable to the printer port. This may cause unexpected problems. See Section 6. Perform the operational check. Unpack the Multiskan EX instrument and accessories carefully with the arrows on the transport package pointing upwards. To lift the instrument, put your fingers under the bottom on both sides and lift it with your back straight.

Caution: When unpacking the instrument, it is recommended that two people lift the instrument together, taking proper precautions to avoid injury. Retain the original packaging and packing material for future transportation. The packaging is designed to assure safe transport and minimize transit damage.

Also retain all instrument-related documentation provided by the manufacturer for future use. If the optional printer has been ordered, it is included uninstalled. Please contact technical service for installation. Paper roll shaft Fig.

If the transport package has been damaged in transit, it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrument. Neither the manufacturer nor its agents can be held responsible for any damage incurred in transit, but the manufacturer will make every effort to help obtain restitution from the carrier.

Upon receipt of the carrier's inspection report, arrangements will be made for repair or replacement. The Multiskan EX does not produce operating noise at a level that would be harmful.

No sound level measurements are required after installation. Warning: 5. Wash your hands thoroughly after handling test fluids. Observe normal laboratory procedures for handling potentially dangerous samples. Use proper protective clothing. Use disposable gloves. Ensure that the working area is well ventilated.

Never spill fluids in or on the equipment. Only use the instrument for the purpose it is intended for. Refer to Section 3. Unpack the instrument. Remove the two 2 instrument cover retaining screws Fig. Install the filter wheel and the lamp.

See Sections 5. Connect the printer or the computer according to the requirements. Plug into mains. Setup the instrument. Unpack the filter wheel. It is delivered in a filter wheel box. Check that all filters are clean and undamaged. Position slot Fig. Caution: When installing the filter wheel and the lamp, do not touch any other mechanical or electronic part. Place the filter wheel into the filter slot Fig. A magnet locking mechanism will automatically lock the wheel in the correct position and the optical filter position sensor will make sure that the correct filter is used during measurement.

Filter slot Fig. Turn the wheel over. Unpack the lamp. Release the terminal socket wire from its transportation holder located inside the instrument. Hold the terminal socket vertically and the lamp so that the claw is pointing upwards.

Fit the lamp contacts to the terminal socket Fig. Press the lamp tightly to fit it properly Fig. Caution: When fitting the lamp contacts, be careful not to bend the contacts. This might cause the bulb to detach from the lamp. Warning: If the instrument has been in use and you need to replace a burned lamp, the lamp can be very hot.

Wait for the lamp to cool down before replacing it. Terminal socket Lamp contacts Fig. Place the lamp in its place so that the claw in the lamp fits in the groove Fig. Push the lamp all the way down. Visually check that there are no loose parts inside the instrument. Close the instrument cover and replace the two cover retaining screws. However, each manufacturer's plates have slightly different dimensions. The plate carrier can therefore be adjusted to suit the used plate type.

When the Multiskan EX is used for shaking the samples, if there is even a small gap, the plate may spill the contents. This is especially important when using robots. The plate may otherwise stay tilted, especially when using robots.

Adjust the plate carrier using the screws on the right side Fig. Ball screws Fig. Never use a mains supply cable other than the Thermo Scientific mains supply cable designed for your region. Make sure that the protective conductor inside or outside the instrument is never interrupted and that the protective conductor terminal is not disconnected. Ensure that the mains switch Fig. Connect the mains supply cable to the mains input socket Fig.

Connect the instrument to a correctly installed line power outlet that has a protective conductor that is grounded. Data transfer to a printer may occur via a built-in RSC interface or via a parallel Centronics interface. The configuration is specified as for data terminal equipment DTE. You can select the baud rate i.

The following baud rates are available: ; ; ; ; ; ; , and The baud rate is set by the manufacturer. The character format is 10 bits with 1 start bit, 8 data bits, 1 stop bit and no parity. The format cannot be changed. The handshake type used is set by the manufacturer. You can select the desired alternative through a DIL switch at the rear of the instrument see Section 5. You can select the desired configuration through the DIL switches at the rear of the instrument see Section 5.

The following configuration is set by the manufacturer: pin 2 transmits and pin 3 receives. The factory set DIL switch positions are given in Fig.

The position 4 in the secondary interface DIL switches Fig. The positions 5 to 8 in the secondary interface DIL switches are not in use and it is recommended that they are in down position. Consult the operating manual supplied with the computer or printer 2. Contact the dealer from which the computer or printer was purchased 3. Contact the computer or printer manufacturer.

The low negative state indicates that the printer or computer is busy and not ready to accept a data character. Thus before sending each character, the Multiskan EX waits until the other device sets the DSR line to the high state.

The configuration is selected through the DIL switches 5, 6, 7 and 8 Fig. The input tells the Multiskan EX that the printer or computer is ready. GND, signal ground Note: Operate the instrument only with software and hardware specifically designed or selected for it.

The instrument does not verify the logic flow of the received commands. Thermo Fisher Scientific assumes no liability for the use of third-party software applications. Computer control takes place via the RSC interface.

Start the computer mode by selecting measurement mode 6 computer control. Press either the stop key or send a Q command to return to keyboard use.

F8 Filter selection FMnn A new R command has to be entered now in order to return to the computer control. This speed is selected as default. Each command must be terminated by LF line feed. The Multiskan EX disregards spaces before the command. Up to 48 extra characters between the command and LF will be ignored. Error see Section8 Troubleshooting.

An invalid command that the Multiskan EX was not able to understand. The P and PM commands are acknowledged by sending values for the measured wells. Y where X can be The T command is acknowledged by sending XX. MAR X, where X can be Connect the printer.

Refer to 6. If the printer is not in use, check that the printer settings are set to off. Switch the instrument on using the power switch at the rear of the instrument Fig. When the instrument is switched on, wait about one 1 minute for the instrument to warm up.

Start the preinstalled measurement program in the instrument memory: Press start 4. Check that the plate carrier moves under the measurement assembly cover Fig. No error messages should appear on the display. Note: The printer destination is set to external printer as default.

Follow the instructions presented in Section 6. If you want to perform a verification of the instrument performance, a Thermo Scientific Multiskan Verification plate, Cat. Table 6. You can also use the number keys to directly select the desired parameter.

Indicates the actions you should take. You find a table showing all programming parameters and a detailed description of the different measurements and calculations in Section 6. Select the program module.

Select the measurement mode and measurement parameters. Select the calculation mode and calculation parameters. You have now made a program. Save it to the instrument memory. It is recommended to keep a record of stored programs. You can use Table 6. Switch the instrument on To prolong the useful life of the lamp, switch off the instrument when it is not in use.

Wait one 1 minute Select first the program module. Take the stored program into use. Check that the filter and the plate are correct and that the plate is placed properly on the plate carrier Fig.

Place the microplate onto the plate carrier It is recommended that the assay include internal quality controls. The instrument performs the measurement. After the measurement, the results are printed on the internal printer or sent via the interface and recorded on the external printer or the computer, according to the selection see Section 6. Check from the printout that the used general and measurement parameters were as intended. Remove the microplate from the plate carrier Caution: Handle the microplate with care to avoid any contamination of the instrument and therefore subsequent specimens.

The contamination of the specimen leads to incorrect results. If the contents of the microplate do spill, however, follow the decontamination procedure given in Section 7.

Follow also the system supplier's instructions in regard to the external PC software, reagent kit and specimen handling in the event of contamination. Warning: If the instrument is used in a manner not specified by the manufacturer, the protection provided by the instrument may be impaired.

See Section 9. Table of Contents. Thermo Scientific. Previous Page. Next Page. Page 2 Warranty The Thermo Scientific Microplate Instrumentation products are fully guaranteed against defective parts and materials, including defects caused by poor workmanship for a period of one year from the date of delivery.

Read the manual in its entirety before operating the instrument. Page 4 These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as indicated. Safety symbols and markings used on the Multiskan FC The following symbols and markings appear on the type label and the instrument itself. Page 5 Warning and other markings used in the documentation The following symbols and markings appear in this user manual.

Risk of electric shock. Biohazard risk. Hot plate, risk of burns. Risk of injury to the user s. Risk of damage to the instrument, other equipment or loss of performance or function in a specific application. Page 7 Results in list format Page 8 Chapter Ordering Information The Multiskan FC is part of the analyzing system used by the end user, who therefore is responsible for validation of the whole system for reliable and safe results.

Unpacking How to unpack Move the packed instrument to its site of operation. Environmental requirements When you set up your Multiskan FC, avoid sites of operation with excess dust, vibrations, strong magnetic fields, direct sunlight, draft, excessive moisture, or large temperature fluctuations. Page 12 Figure 4. Unfastening the transport lock 3. Pull the transport lock bar out Figure 5. Figure 5. Pulling the transport lock out 4.

Keep the transport lock bar and tag Figure 6 for future relocation or transportation of the instrument. Page Installing The Filter Wheel 1. Unpack the filter wheel Figure 7.

It is delivered in a filter wheel box. Check that all filters are clean and undamaged. Position slot Figure 7. Multiskan FC filter wheel Caution! Do not touch the filters with your bare hands.

Connecting the power supply cable Connecting to a computer If you are using a computer with the Multiskan FC, connect the communication cable to the USB port for the computer Figure 9. Connecting to a printer If you are using a printer, connect it to the USB port for the printer Figure 9.

The external USB Page Switching On Settings menu. Operate the instrument only with software and hardware that is specifically designed or selected for it. Display Lamp and filter wheel Incubator The optional incubator is only available with the Multiskan FC with incubator model.

The incubator chamber consists of thermal elements on the top and bottom of the incubator chamber. The upper electrical thermal element prevents condensation on the microplate that is being incubated. The keys also have other functions depending on the level in the internal software.

Depending on the level of the internal software, the FILE key Page 21 The clock on the Main tab shows the real time. The Incubation icon also shows the current temperature only Multiskan FC with incubator model. See the info text bar for the required actions of the F1-F3 keys.

The action text on the info text bar changes according to the level of the internal software. Protocol The Protocol row in the Main menu shows the name of the active protocol. Page Plate Format Parameters Note! It is recommended to back up the protocols regularly. The backup is created by exporting the protocols to the USB memory device. If you make any changes to the processing parameters of a measured run in the Processing menu, the changes are made to that run only.

Page Measurement Parameters Measurement parameters You can define the measurement filter s , number of readings, and measurement mode in the Measurement window. Filters Filter 1 defines the first main filter wavelength. Filter 2 defines the second reference filter wavelength.

You can select the suitable filter from the list of available filters. You must physically install the filter into the filter wheel, as well as introduce the additional filter to the internal software of the instrument in the Settings window.

Page Incubation Parameters Incubation parameters Note! Incubation is only available if the instrument configuration supports incubation. You can define the incubation parameters, incubation time, and temperature in the Incubation window. The Incubation icon and row is visible in the Main menu of the instruments with an incubator indicating the present temperature. Plate layout parameters You can set the plate map of the protocol in the Plate layout window. The calculations of the results are made based on the plate layout.

The plate layout parameters have to be set prior to measurement. Page Preprocessing Parameters Note! Blank subtraction is made automatically if the plate layout includes blanks. The blank used in the calculations is always an average of the blanks included in the layout. You can determine the sample type in Well type. Page 28 Preprocessing is used with dual two wavelength measurements, for example, to subtract the reference wavelength data in dual measurements and with kinetic measurements to reduce data in kinetic calculations.

Table 8. Page Kinetic Calculations Kinetic calculations Kinetic calculations can be used when the number of readings is more than 1 in the measurement parameters see Measurement parameters. Also the results of a kinetic calculation can be used as source data for further calculations. Page 31 Figure Page 32 1 to 3, the second rate using the measurements 2 to 4, and so on up to measurements 8 to The maximum rate will be the maximum value among these calculated rates. Time to maximum rate The time to maximum rate is calculated similarly to the maximum rate, but the result is reported as the time in seconds from the first reading to the point on the line where the maximum rate occurs instead of the rate itself.

Page 33 First define the base value by entering the number of readings from the start in baseline. The base value is the average of results of the given baseline count from the beginning or end of results. Time to maximum The time to maximum is used to calculate the time elapsed before the maximum measurement signal in each well is reached.

Page Curve Fits Note! The plate layout must include calibrators and corresponding calibrator parameters, such as concentrations, to enable them for further calculations, for example, curve fits.